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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos
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Capitoli
- 00:05Titolo
- 01:05Bleach and Dechorionate 48 hpf Zebrafish Embryos
- 03:19Embryos for Dissociation
- 05:15Preparation of Dissociated Embryos
- 06:21Preparation of Methylcellulose
- 07:30Addition of Dissociated Embryos to Methylcellulose
- 08:53Results: Quantitation of Zebrafish Embryonic Hematopoietic Stem and Progenitor Cells
- 09:32Conclusion
Here, we present a simple method to quantitate hematopoietic stem and progenitor cells (HSPCs) in embryonic zebrafish. HSPCs from dissociated zebrafish are plated in methylcellulose with supportive factors, differentiating into mature blood. This allows the detection of blood defects and allows drug screening to be easily conducted.
