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定量实时反转录 PCR 技术对猕猴猴血浆 MicroRNA 水平的绝对量化

JoVE Journal
Biologia

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JoVE Journal Biologia

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

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定量实时反转录 PCR 技术对猕猴猴血浆 MicroRNA 水平的绝对量化

DOI:

10.3791/56850-v

•

10:23 min

•

February 12, 2018

•

Takuma Iguchi, Noriyo Niino, Satoshi Tamai, Ken Sakurai, Kazuhiko Mori

¹Medicinal Safety Research Laboratories,Daiichi Sankyo Co., Ltd.

Capitoli

00:04Titolo
01:01RNA Extraction
03:43cDNA Synthesis
04:55Pre-amplification
06:02Quantitative Real-time PCR
07:05Data Analysis
07:27Results: Use of Quantitative Real-time Reverse Transcription PCR with or without Pre-amplification to Measure the Absolute Levels of Plasma micro-RNAs
09:54Conclusion

Summary

Traduzione automatica

本报告描述了一种测量血浆 miRNA 绝对水平的协议, 使用定量的实时反向转录 PCR 或不预放大。该议定书对血浆 miRNAs 的数量有了更好的了解, 并允许对来自不同研究或实验室的相应数据进行定性评估。

Tags

Keywords: Plasma MicroRNA Absolute Quantification Quantitative Real-time Reverse Transcription PCR Cynomolgus Monkeys RNA Extraction Synthetic MicroRNA Biomarkers Translational Research

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