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Generation and Culturing of Primary Human Keratinocytes from Adult Skin
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

DOI:
10.3791/56863-v

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10:42 min

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December 22, 2017

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1Department of Dermatology,Aarhus University Hospital

Capitoli

  • 00:05Titolo
  • 00:52Isolation of Keratinocytes from Human Skin
  • 05:32Passaging of Keratinocytes
  • 07:32Freezing Keratinocytes
  • 08:48Results: Primary Human Keratinocyte Culture from Adult Skin
  • 10:06Conclusion

Summary

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The human skin acts as a first line of defense against the external environment. We present a method for isolating primary human keratinocytes from adult skin. These isolated keratinocytes are useful in numerous experimental setups, and are a highly suitable model for studying molecular mechanisms in cutaneous biology in vitro.

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Keywords: Primary Human KeratinocytesAdult SkinEpidermal Cell BiologySkin DiseasesTrypsinDPBSKSFMRPMI 1640FBSGentamicinEpidermal CellsDermal Compartment

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