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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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JoVE Journal Ambiente
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

DOI:
10.3791/56891-v

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14:15 min

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February 23, 2018

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, , , , , ,
1Department of Plant Pathology,Washington State University, 2Parma Research and Extension Center,University of Idaho

Capitoli

  • 00:00Titolo
  • 01:06Magnetic bead-based DNA extraction
  • 02:06Soil lysis
  • 11:44Representative Results
  • 13:24Discussion
  • 13:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

Rapid and accurate detection of plant pathogens on-site, especially soil-borne pathogens, is crucial to prevent further inoculum production and proliferation of plant diseases in the field. The method developed here using a portable real-time PCR detection system enables on-site diagnosis under field conditions.

Tags

On-site DetectionSoil-borne PhytopathogensPortable Real-time PCRMagnetic Bead-based DNA ExtractionPotato PathogensSpongospora SubterraneaField DiagnosisPlant Pathogen Detection

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