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Single-cell Microfluidic Analysis of Bacillus subtilis
 

Single-cell Microfluidic Analysis of Bacillus subtilis

Article DOI: 10.3791/56901-v • 10:37 min • January 26th, 2018
January 26th, 2018 •
1, 2
1Department of Microbiology and Molecular Genetics, Oklahoma State University, 2Department of Molecular and Cellular Biology, Harvard University

Capitoli

Riepilogo
Automatic Translation

We present a method for the microfluidic analysis of individual bacterial cell lineages using Bacillus subtilis as an example. The method overcomes shortcomings of traditional analytical methods in microbiology by allowing observation of hundreds of cell generations under tightly controllable and uniform growth conditions.

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Keywords: Microfluidic Bacillus Subtilis Single-cell Analysis Cell Lineage Long-term Observation PDMS Master Oxygen Plasma Treatment Cover Glass
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