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Simpel Generation af højtydende kultur af inducerede neuroner fra menneskelige voksen hud fibroblaster
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1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center,Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute,University of Cambridge
Capitoli
- 00:04Titolo
- 01:02Preparation of Skin Fibroblasts for Reprogramming
- 01:43Plating for Reprogramming (Day 1)
- 03:22Viral Transduction (Day 0)
- 04:12Maintenance of the Converting Cells
- 04:58FACS
- 07:33Results: Generation of Induced Neurons from Adult Human Dermal Fibroblasts
- 08:15Conclusion
Direkte neuronal omprogrammering genererer neuroner, der opretholder en alder af det udgangspunktet i somatiske celler. Her, beskriver vi en enkelt vektor-baseret metode til at generere induceret neuroner fra dermal fibroblaster fremstillet af voksne menneskelige donorer.
