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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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JoVE Journal Biologia dello sviluppo
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

DOI:
10.3791/56919-v

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05:48 min

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January 02, 2018

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,
1Department of Molecular and Human Genetics,Baylor College of Medicine, 2Program in Developmental Biology,Baylor College of Medicine

Capitoli

  • 00:05Titolo
  • 00:38Assessment of Binding Between Notch Receptor and Trans-ligands
  • 01:47Aggregation Between Signal-sending and Signal-receiving Cells
  • 03:11Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
  • 04:15Results: Faithful Analysis of Notch Signaling
  • 05:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.

Tags

Keywords: Cell Aggregation AssayNotch ReceptorTrans-ligandsCis-ligandsDrosophilaS2 CellsLigand BindingCell SignalingAggregation QuantificationMicroscopyHemacytometer

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