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セルショウジョウバエ切り込みをトランスとの結合を評価する集計の試金-配位子とCisによるその阻害-配位子
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
DOI:

05:48 min

January 02, 2018

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Capitoli

  • 00:05Titolo
  • 00:38Assessment of Binding Between Notch Receptor and Trans-ligands
  • 01:47Aggregation Between Signal-sending and Signal-receiving Cells
  • 03:11Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
  • 04:15Results: Faithful Analysis of Notch Signaling
  • 05:30Conclusion

Summary

Traduzione automatica

-トランスシスによる活性化と Notch 受容体の阻害の区別が困難な体内システムの複雑さ-配位子、それぞれ。ここでは、トランスショウジョウバエノッチのバインディングの定性・半定量評価のための in vitro細胞凝集アッセイに基づくプロトコルを提案する-配位子 vs cis-配位子。

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