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Neuron-Macrophage co culturen te activeren van macrofagen afscheidende moleculaire factoren met Neurite uitgroei activiteit
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
DOI:

08:52 min

March 30, 2018

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Capitoli

  • 00:04Titolo
  • 01:06Culture Preparation of Dissociated Adult DRG Neuron
  • 03:41Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
  • 05:47Neurite Outgrowth Assay with Collected Conditioned Medium
  • 07:05Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
  • 07:55Conclusion

Summary

Traduzione automatica

Het huidige protocol presenteert experimentele procedures ter stimulering van gekweekte macrofagen te worden begiftigd met capaciteit om moleculaire factoren ter bevordering van de uitgroei van de neurite vrij te geven. Behandeling van kamp tot de neuron-macrofaag co culturen induceert de macrofagen produceren geconditioneerde medium dat sterke neurite uitgroei activiteit bezit.

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