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Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
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JoVE Journal Ricerca sul cancro
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

DOI:
10.3791/56943-v

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11:20 min

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March 21, 2018

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, , ,
1Genome Analysis Center,Yamanashi Central Hospital, 2Pathology Division, Laboratory Department,Yamanashi Central Hospital, 3The University of Tokyo

Capitoli

  • 00:04Titolo
  • 00:37TIC Preparation for Quick Microscopic Assessment
  • 02:04PEN Membrane Slide Film Preparation for Genetic Testing
  • 02:40Next Generation Sequencing
  • 09:24Results: TIC DNA Suitable for Range of Genetic Testing
  • 10:57Conclusion

Summary

Traduzione automatica

Traduzione automatica

Obtaining high-quality genomic DNA from tumor tissues is an essential first step for analyzing genetic alterations using next generation sequencing. In this article, we present a simple and rapid method to enrich tumor cells and obtain intact DNA from touch imprint cytology specimens.

Tags

Tumor DNATouch Imprint CytologyClinical-pathological SpecimensHigh-quality DNARapid DNA ExtractionRicerca sul cancroDiagnosisTreatmentDNA ExtractionMicroscopic AssessmentDNA Sequencing

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