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用触印细胞学方法快速获得临床病理标本中高质量肿瘤 DNA

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Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

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用触印细胞学方法快速获得临床病理标本中高质量肿瘤 DNA

DOI:

10.3791/56943-v

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11:20 min

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March 21, 2018

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Kenji Amemiya², Yosuke Hirotsu, Toshio Oyama, Masao Omata³

¹Genome Analysis Center,Yamanashi Central Hospital, ²Pathology Division, Laboratory Department,Yamanashi Central Hospital, ³The University of Tokyo

Capitoli

00:04Titolo
00:37TIC Preparation for Quick Microscopic Assessment
02:04PEN Membrane Slide Film Preparation for Genetic Testing
02:40Next Generation Sequencing
09:24Results: TIC DNA Suitable for Range of Genetic Testing
10:57Conclusion

Summary

Traduzione automatica

从肿瘤组织获得高质量的基因组 DNA 是利用下一代测序分析基因改变的重要第一步。本文提出一种简便、快速的方法来丰富肿瘤细胞, 从接触印记细胞学标本中获得完整的 DNA。

Tags

Keywords: Tumor DNA Touch Imprint Cytology Clinical-pathological Specimens High-quality DNA Rapid DNA Extraction Ricerca sul cancro Diagnosis Treatment DNA Extraction Microscopic Assessment DNA Sequencing

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