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Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
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Biologia
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JoVE Journal Biologia
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

DOI:
10.3791/56955-v

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05:53 min

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May 29, 2018

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, , ,
1Knock Out Mouse Core,NIEHS/NIH

Capitoli

  • 00:05Titolo
  • 00:44Embryo Collection
  • 02:01Embryo Injection and Transfer
  • 04:26Results: Representative Post-embryonic Stem (ES) Cell Transfer Pregnancy, Survival, and Chimersim Rates
  • 04:59Conclusion

Summary

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Here we present a protocol to increase chimera production without the use of new equipment. A simple orientation change of the embryo for injection can increase the number of embryos produced, and potentially reduce the timeline to germline transmission.

Tags

Embryonic Stem CellsChimeraTrans-inner Cell Mass InjectionBlastocystMicroinjectionCell LineCell CultureBiologistGraduate StudentMicroscopyLaboratory Techniques

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