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Immunology and Infection

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JoVE Journal Immunology and Infection
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
 

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Article DOI: 10.3791/56957-v • 08:08 min • January 11th, 2018
January 11th, 2018 •
1, 1
1Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health

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This technique describes an automated batch image processor designed to measure polysaccharide capsule and body radii. While initially designed for Cryptococcus neoformans capsule measurements the automated image processor can also be applied to other contrast based detection of circular objects.

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Keywords: Automated Measurement Cryptococcal Species Polysaccharide Capsule Cell Body Virulence India Ink Slide H99 Strain Bright-field Microscope Measurement Algorithm QCA.py
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