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单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

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单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析

DOI:

10.3791/56973-v

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09:32 min

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May 08, 2018

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Brandon K. Hadland², Barbara Varnum-Finney, Cynthia Nourigat-Mckay, David Flowers, Irwin D. Bernstein²

¹Clinical Research Division,Fred Hutchinson Cancer Research Center, ²Department of Pediatrics, Division of Pediatric Hematology/Oncology,University of Washington School of Medicine

Capitoli

00:05Titolo
00:52Murine Embryonic Cell Staining
02:49Single Hemogenic Precursor Index Sorting
04:44Clonal Hematopoietic Progeny and Individual Clone Engraftment Property Analyses
07:02Results: Representative Embryonic Hematopoietic Stem Cell Precursor Analysis
09:08Conclusion

Summary

Traduzione automatica

本文介绍了小鼠胚胎发育过程中造血干细胞前体的克隆分析方法。我们结合内皮细胞共培养和移植对胚胎主动脉-性腺-中区单细胞的指标进行分类, 以表征单造血前体的表型性质和植入电位。

Tags

Keywords: Clonal Analysis Embryonic Hematopoietic Stem Cell Precursors Single Cell Index Sorting Endothelial Cell Niche Co-culture Developmental Hematopoiesis Lineage Contributions Hemogenic Precursors Phenotypic Properties HSC Precursors Collagenase Antibody Cocktail Flow Cytometry Single Cell Sorting Index Sorting

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