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Primary Cell Cultures from the Mouse Retinal Pigment Epithelium
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Biologia
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JoVE Journal Biologia
Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

DOI:
10.3791/56997-v

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09:35 min

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March 16, 2018

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, , , ,
1Department of Ophthalmology,University of Pittsburgh School of Medicine, 2Wilmer Eye Institute,The Johns Hopkins University School of Medicine

Capitoli

  • 00:04Titolo
  • 00:50Isolating and Preparing Eyeballs for Dissection
  • 03:18Isolating the RPE-choroid-sclera Complex from Eyeballs
  • 05:07Collecting and Culturing Primary RPE Cells from RPE-choroid-sclera Complexes
  • 07:22Results: Characterize Mouse Primary RPE Cultures
  • 08:59Conclusion

Summary

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Traduzione automatica

The retinal pigment epithelium (RPE) is a multi-functional epithelium of the eye. Here we present a protocol to establish primary cell cultures derived from the murine RPE.

Tags

Primary Cell CultureMouse Retinal Pigment EpitheliumRPE IsolationRPE Cell CultureEuthanasiaEye DissectionDispase IILaminar Flow CabinetGrowth MediumMicroscopy

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