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Immunologia e infezioni

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双光子激光显微镜在细胞膜修复中的应用

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Cell Membrane Repair Assay Using a Two-photon Laser Microscope

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双光子激光显微镜在细胞膜修复中的应用

DOI:

10.3791/56999-v

•

06:35 min

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January 02, 2018

•

Joshua J. A. Lee, Rika Maruyama, Hidetoshi Sakurai, Toshifumi Yokota

¹Department of Medical Genetics,University of Alberta Faculty of Medicine and Dentistry, ²Center for iPS Cell Research and Application,Kyoto University

Capitoli

00:05Titolo
00:38Full-length Dysferlin (DYSF) Plasmid Cell Transfection
03:09Two-photon Laser Wounding Assay
05:19Results: Representative Fluorescence Quantification Over Time
06:00Conclusion

Summary

Traduzione automatica

双光子激光的细胞膜损伤是一种广泛应用的评价膜加盖能力的方法, 可应用于多种细胞类型。在这里, 我们描述了一个协议的体外活成像膜加盖在 dysferlinopathy 患者细胞后双光子激光消融。

Tags

Cell Membrane Repair Muscular Dystrophy Two-photon Laser Microscope Fibroblast Cells Plasma Membrane Membrane Resealing Dysferlin Transfection FM4-64 Dye Two-photon Wounding Assay

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