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肉腫患者由来異種移植片からの腫瘍始発細胞の単離と特徴付け
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
DOI:

07:18 min

June 13, 2019

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Capitoli

  • 00:04Titolo
  • 00:44Sarcoma Tissue Sample Processing and Patient-derived Xenograft (PDX) Formation
  • 02:32Tumor-initiating Cell (TIC) Sorting and Sarcosphere Formation
  • 05:11Results: Representative Human Leukocyte Antigen (HLA)-1-Negative TIC Characterization
  • 06:42Conclusion

Summary

Traduzione automatica

蛍光活性化細胞選別によるヒト肉腫患者由来異種移植片からの腫瘍開始細胞の単離に関する詳細なプロトコルを説明し、ヒト白血病抗原-1(HLA-1)を陰性マーカーとして用い、さらなる検証を行う。これらのHLA-1陰性腫瘍始発細胞の特徴付け。

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