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单细胞分辨荧光活成像在幼虫脑培养中的果蝇昼夜节律时钟

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

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单细胞分辨荧光活成像在幼虫脑培养中的果蝇昼夜节律时钟

DOI:

10.3791/57015-v

•

07:05 min

•

January 19, 2018

•

Virginie Sabado, Emi Nagoshi

¹Department of Genetics and Evolution,University of Geneva

Capitoli

00:05Titolo
00:40Preparations and Dissection
02:48Embedding Brain Explants in a 3D Matrix and Mounting
04:56Results: Long-term Recordings of Circadian Fluorescent Reports in Ex Vivo Larval Brain Cultures
06:22Conclusion

Summary

Traduzione automatica

该协议的目的是建立活体果蝇幼虫脑培养优化监测昼夜节律分子节律与长期荧光时间推移成像。并对该方法在药理实验中的应用进行了探讨。

Tags

Single-cell Fluorescence Live Imaging Drosophila Circadian Clocks Larval Brain Culture Neurogenetics Gene Expression Fluorescent Probes SAM Medium Thrombin Penicillin Streptomycin Fibrinogen Dissection Forceps DSS Third Instar Larvae Brain Transfer Embedding Procedure

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