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活完好斑马鱼的单细胞 Photoconversion
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Single-cell Photoconversion in Living Intact Zebrafish
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活完好斑马鱼的单细胞 Photoconversion

DOI:
10.3791/57024-v

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09:49 min

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March 19, 2018

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,
1Department of Biological Sciences,University of Notre Dame, 2Center for Stem Cells and Regenerative Medicine,University of Notre Dame

Capitoli

  • 00:05Titolo
  • 00:39Preparation of Zebrafish Specimen
  • 02:12Microscope Mounting and Pre-conversion Imaging
  • 03:35Single-cell Photoconversion
  • 06:18Post-photoconversion Imaging
  • 07:38Results: Photoconverted Eos Protein in Single and a Population of Ganglion Cells in Transgenic Zebrafish
  • 08:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个协议, 以显示如何实现细胞 photoconversion 是通过紫外线照射到特定区域表达荧光蛋白, Eos, 在活体动物。

Tags

Keywords: Single-cell PhotoconversionZebrafishFluorescent Cell PopulationsDevelopmental QuestionsSingle-cell MigrationMinimally InvasivePhotoconvertible Protein488 Nanometer Light SourceGFP Filter SetsDechorionateLow Melting Point AgaroseAnesthetized Transgenic Sox10 Eos Positive FishConfocal MicroscopyPre-conversion ImagingDorsal Root GanglionC488 Laser3D Capture

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