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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

DOI:
10.3791/57033-v

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10:21 min

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February 21, 2018

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, , ,
1Department of Hemato-Oncology,Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine,Charles University

Capitoli

  • 00:04Titolo
  • 00:58Media Preparation
  • 01:24Production of Retrovirus
  • 03:28Tritation of Virus Containing a Puromycin Reporter
  • 05:04Transduction of 32D/G-CSF-R Cells
  • 06:1032D/G-CSF-R Cell Differentiation Assay
  • 07:32Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media
  • 09:21Conclusion

Summary

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Here detailed protocols for culturing the murine myeloid precursor 32D/G-CSF-R cell line, performing viral infections, and carrying out proliferation and differentiation assays are presented. This cell line is suitable for studying myeloid cell development, and the role of genes of interest in myeloid cell growth and neutrophilic differentiation.

Tags

Myeloid Precursor Cells32D-G-CSF-R CellsProliferationDifferentiationGenetic ModificationRetroviral TransductionBosc23 CellsRPMI-1640 MediumInterleukin-3MSCV Retroviral ConstructPCL Echo Packaging VectorPolyethylenimineDMEM MediumFetal Bovine SerumVirus ProductionVirus Titration

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