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En Time-lapse, etiket-fri, kvantitative fase Imaging undersøgelse af passive og aktive menneskelige kræftceller
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
DOI:

12:48 min

February 16, 2018

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Capitoli

  • 00:04Titolo
  • 00:58Cell Preparation
  • 02:35Cell Preparation: Splitting KHOS-A and KHOS-N Cells
  • 03:50Cell Preparation: Seeding KHOS-A and KHOS-N Cells for QPI
  • 04:30Quantitative Phase Image Acquisition: Cover Slip Set-up
  • 05:19Quantitative Phase Imaging
  • 07:03Analyzing Cell Morphology
  • 08:20Analyzing Cell Proliferation
  • 08:54Analyzing Cell Motion
  • 10:16Results: Quantiative Phase Imaging of Dormant and Active Human Cancer Cells
  • 11:50Conclusion

Summary

Traduzione automatica

Passive og aktive kræft celle fænotyper var karakteriseret ved hjælp af kvantitative fase billeddannelse. Celle spredning, migration og morfologi assays blev integreret og analyseres i en simpel metode.

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