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Trichostatin a 和维生素 C 的组合治疗提高体细胞细胞核移植克隆小鼠的效率
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
DOI:

09:52 min

April 26, 2018

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Capitoli

  • 00:05Titolo
  • 00:52Preparation of Deionized Bovine Serum Albumin
  • 01:48Oocyte Collection
  • 03:26Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes
  • 05:32Fusion of Donor Cell and Enucleated Oocyte
  • 07:42Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C
  • 08:26Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment
  • 09:15Conclusion

Summary

Traduzione automatica

我们用 trichostatin a、维生素 C 和去离子牛血清白蛋白来描述一种显著改进的小鼠克隆方法。我们展示了一个简化的、可重现的协议, 支持克隆胚胎的高效开发。因此, 该方法可以成为鼠标克隆的标准化程序。

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