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Combinacional tratamento de tricostatina A e a vitamina C melhora a eficiência da clonagem de ratos por transferência Nuclear de células somáticas
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1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology,Kindai University, 2Faculty of Biology-Oriented Science and Technology,Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology,University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture,Kyoto University, 5Institute of Advanced Technology,Kindai University
Capitoli
- 00:05Titolo
- 00:52Preparation of Deionized Bovine Serum Albumin
- 01:48Oocyte Collection
- 03:26Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes
- 05:32Fusion of Donor Cell and Enucleated Oocyte
- 07:42Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C
- 08:26Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment
- 09:15Conclusion
Nós descrevemos um método melhorado dramaticamente para clonagem de rato usando tricostatina A, vitamina C e desionizada albumina de soro bovino. Mostramos um protocolo simplificado, reprodutível, que suporta o desenvolvimento eficiente de embriões clonados. Assim, este método pode tornar-se um procedimento padronizado para clonagem de rato.
