Rivista
/
Neuroscienze
/
An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

DOI:
10.3791/57038-v

•

09:35 min

•

February 26, 2018

•

, , ,
1Institute of Neurology (Edinger Institute),Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology,Goethe University Hospital

Capitoli

  • 00:04Titolo
  • 01:15Administration of Tracers
  • 02:01Blood Collection and Cardiac Perfusion
  • 03:36Organ Collection and Storage
  • 05:01Homogenization and Centrifugation
  • 06:38Immunofluorescence Visualization of Tracer
  • 07:59Results: Tracer Clearance at Short and Long Circulation Times
  • 08:51Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.

Tags

Keywords: In VivoBlood-brain BarrierPermeabilityFluorescent TracersMiceNeurological DisordersBrain EdemaBlood-brain Barrier DysfunctionCardiac PerfusionFluorometryFluorescence Microscopy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/57038?article_type=v

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
check_url/it/57038?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/it/57038?article_type=v

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
check_url/it/57038?article_type=v

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease
check_url/it/57038?article_type=v

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke
check_url/it/57038?article_type=v

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model
check_url/it/57038?article_type=v

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
check_url/it/57038?article_type=v

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
check_url/it/57038?article_type=v

A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model (Video) | JoVE
check_url/it/57038?article_type=v

Applications of TEER Detection to Assess Cell Barrier Integrity (Video) | JoVE

Read Article