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血管血管和血管生成的动力学分析体外
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro
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血管血管和血管生成的动力学分析体外

DOI:
10.3791/57044-v

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11:03 min

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January 31, 2018

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, , ,
1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
  • 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
  • 08:12Results: Representative Kinetic Analysis of Vasculogenesis
  • 10:22Conclusion

Summary

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在这里, 我们提出了一个时间推移成像和分析血管在体外使用相衬显微镜结合开放源码软件, 动力学分析的血管。本协议可用于定量评估多种细胞类型的血管电位或模型血管疾病的实验条件。

Tags

VasculogenesisAngiogenesisKinetic AnalysisLive-cell ImagingQuantitateVessel FormationNetwork Structural PatternsCell PopulationPathologic StateMicroscope Stage Top IncubatorCarbon DioxideHumidityLive-cell ChamberChambered SlideBasement Membrane MatrixMatrigelPipetting

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