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High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds
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Biologia
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JoVE Journal Biologia
High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

DOI:
10.3791/57053-v

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12:32 min

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January 23, 2018

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, , ,
1Nutrition, Dietetics and Food Science Department,Brigham Young University

Capitoli

  • 00:05Titolo
  • 00:54Cell Culture
  • 01:56Preparation of Cells for High-resolution Respirometry
  • 03:40High-resolution Respirometry
  • 10:08Results: Evaluation of the effects of Monomeric Cocoa Epicatechin or Curcumin on Intact Beta Cell Respiration
  • 11:12Conclusion

Summary

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The goal of this protocol is to measure the effect of glucose-mediated changes to mitochondrial respiration in the presence of natural compounds on intact 832/13 beta cells using high-resolution respirometry.

Tags

Keywords: High-resolution RespirometryMitochondrial FunctionPancreatic Beta CellsNatural CompoundsInsulin Stimulatory ConditionsRespiratory FunctionINS-1-derived 832/13 Beta CellsEpicatechinLow Glucose Secretion Assay Buffer

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