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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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Capitoli
- 00:00Titolo
- 00:09Introduction
- 01:03Protocol
- 01:07Introducing the Stop Codon into the Target Gene
- 02:58Expressing and Purifying Site-specifically Acetylated Protein
- 06:21Biochemically Characterizing Acetylated Protein
- 09:39Risultati
- 10:37Summary
Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.
