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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
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Neuroscienze
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JoVE Journal Neuroscienze
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

DOI:
10.3791/57065-v

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10:21 min

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February 16, 2018

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, , ,
1The Ritchie Centre,Hudson Institute of Medical Research, 2Department of Obstetrics & Gynaecology,Monash University

Capitoli

  • 00:04Titolo
  • 00:36Enzymatic Digestion
  • 02:52Myelin Debris Removal
  • 05:53Magnetic Activated Cell Sorting
  • 08:01Results: Analysis of Microglia Survival After Inflammatory Insult
  • 08:42Conclusion

Summary

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A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.

Tags

Microglia IsolationDensity Gradient CentrifugationPrimary MicrogliaNeuroinflammationImmunomodulationDrug ScreeningBrain DissectionCell StrainingCell Viability

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