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2维直接随机光学重建显微成像中间丝和微管
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Biologia
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JoVE Journal Biologia
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
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2维直接随机光学重建显微成像中间丝和微管

DOI:
10.3791/57087-v

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14:23 min

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March 06, 2018

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, , ,
1Cell Polarity, Migration and Cancer Unit, UMR 3691, CNRS,Institut Pasteur, 2UTechS Photonic BioImaging (Imagopole) Citech,Institut Pasteur

Capitoli

  • 00:04Titolo
  • 00:54Cell Fixation
  • 02:25Preparing Samples and Image Acquisition
  • 10:10Image Reconstruction
  • 11:50Results: 2-dimensional Direct Stochastic Optical Reconstruction Microscopy of Intermediate Filaments
  • 12:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

该方法的总体目标是给出从样品制备到图像采集和重建的最佳实验条件, 以执行固定细胞中微管和中间丝的2D 双色 dSTORM 图像。

Tags

2D D-STORMIntermediate FilamentsMicrotubulesCytoskeletal NetworksSample PreparationImmunostainingFluorescence MicroscopyImaging BufferAcquisition Parameters

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