/

/

鼠小胶质细胞的分离培养促进无血清培养基动态分支形态学的研究

JoVE Journal
Neuroscienze

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Neuroscienze

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Please note that all translations are automatically generated. Click here for the English version.

鼠小胶质细胞的分离培养促进无血清培养基动态分支形态学的研究

DOI:

10.3791/57122-v

•

12:00 min

•

March 09, 2018

•

Hannah Y. Collins, Christopher J. Bohlen

¹Department of Neurobiology,Stanford University

Capitoli

00:04Titolo
00:41Tissue Collection and Mechanical Dissociation
02:52Myelin Removal
04:07Immunopanning
07:56Spot Coating Tissue Culture/Coverslips
09:22Results: Defined-medium Microglia Cultures Show Ramified Morphology with Dynamic Processes
11:22Conclusion

Summary

Traduzione automatica

由于缺乏对成熟的体内小胶质细胞的特性进行重述的胶质文化模型, 胶质的详细理解的努力受到了阻碍。该协议描述了一种隔离和培养方法, 旨在维持高分支成熟大鼠小胶质细胞在确定培养基条件下的健壮生存。

Tags

Microglia Isolation Culture Serum-free Rodent Morphology CNS Dounce Homogenizer MSB DPBS

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

肖尔自动数字化的神经元形态分析，多尺度

从小鼠胚胎神经管的神经嵴细胞的分离和培养

啮齿动物的中枢神经系统药物直接脑室交货

原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

动态定量感觉测试表征中央疼痛处理

小胶质细胞作为替代生物传感器确定纳米颗粒神经毒性

密度梯度离心法分离小鼠原发胶质细胞的研究

斑马鱼脑幼虫神经元、巨噬细胞和小胶质的分离和 RNA 提取

应用 ImageJ 对免疫组化制备组织显微照片小胶质细胞形态学的定量研究

共培养实验的奥里戈丁细胞和奥里戈丁酸基质的生成

Read Article