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蛋白质组学与染色质相关蛋白杂交捕获对哺乳动物细胞的适应性研究
JoVE Journal
Genetica
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JoVE Journal Genetica
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
DOI:

09:27 min

June 01, 2018

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Capitoli

  • 00:04Titolo
  • 00:44Cell Culture
  • 03:13Lysis and Shearing
  • 04:14Hybridization Capture
  • 06:14Evaluation of Capture Yield by qPCR with Primers for the Target Capture Region of Interest
  • 07:12Results: Hybridization Capture of Chromatin-associated Proteins in Mammalian Cells
  • 08:38Conclusion

Summary

Traduzione automatica

这是一种方法, 以确定新的 DNA 相互作用的蛋白质在特定的靶点, 依赖序列特定捕获的交联染色质的后续蛋白质组分析。不需要事先了解潜在的结合蛋白, 也无需进行细胞修饰。该技术最初是为酵母开发的, 现在已经适应了哺乳动物细胞。

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