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1School of Biological Sciences,The University of Auckland, 2Research School of Biology,The Australian National University, 3Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences,The University of Auckland, 4Department of Plastic Surgery,Counties Manukau District Health Board, 5Department of Surgery, Faculty of Medical and Health Sciences,The University of Auckland, 6Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences,The University of Auckland, 7Maurice Wilkins Centre,The University of Auckland
Capitoli
- 00:00Titolo
- 01:55Inducing the differentiation of human adipose derived stromal cells
- 03:36Staining for differentiation
- 05:53Imaging cells using high content screening microscope
- 08:23Image Analysis: Cell Scoring
- 09:43Image Analysis: Transfluor
- 10:43Representative results
Tradisjonelle metoder for å vurdere adipogenic differensiering er billig og enkel å bruke, men er ikke spesifikke for endringer i genuttrykk. Vi har utviklet en analysen for å kvantifisere mesenchymal celledifferensiering i eldre adipocytter med en slektslinje spesifikk markør. Denne analysen har forskjellige programmer over grunnleggende forskning og klinisk medisin.
