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人诱导多潜能干细胞衍生肝细胞的药物发现方法
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
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人诱导多潜能干细胞衍生肝细胞的药物发现方法

DOI:
10.3791/57194-v

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12:40 min

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May 19, 2018

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, ,
1Department of Regenerative Medicine and Cell Biology,Medical University of South Carolina

Capitoli

  • 00:04Titolo
  • 01:05Culture of Human Induced Pluripotent Stem Cells
  • 03:39Differentiation of Human iPSCs to Hepatocyte-like Cells and Small Molecule Screening
  • 09:34Results: Differentiation of iPSCs and Identification of Small Molecules for Drug Discovery
  • 11:12Conclusion

Summary

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这里提出的协议描述了一个平台, 用于鉴别肝脏疾病的小分子。本文详细介绍了如何将 iPSCs 分化为96井板中具有肝细胞特征的细胞, 并利用细胞筛选出具有潜在治疗活性的小分子。

Tags

Human Induced Pluripotent Stem CellsHepatocytesLiver DiseaseHigh-throughput ScreeningDrug DiscoveryCell DifferentiationEcadDPBSY-27632EDTAM Medium

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