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Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

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Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

DOI:

10.3791/57212-v

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07:18 min

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April 19, 2018

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Xuesong Feng*¹, Faiza Naz*², Aster H. Juan, Stefania Dell’Orso, Vittorio Sartorelli

¹Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, ²Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Capitoli

00:05Titolo
00:41Cryo-embedding of the Tibialis Anterior Muscle
01:46Cryostat Sectioning
02:33Immunofluorescence and Staining
05:27Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining
06:40Conclusion

Summary

Traduzione automatica

卫星细胞的精确识别对于研究其在各种生理和病理条件下的功能至关重要。本文提出了一种基于免疫荧光染色的成人骨骼肌切片卫星细胞识别的协议。

Tags

Keywords: Skeletal Muscle Satellite Cells Immunofluorescence Pax7 Laminin Cryosectioning Cryostat Antigen Retrieval Immunostaining

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