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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

DOI:
10.3791/57223-v

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09:17 min

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March 14, 2018

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, ,
1Center for Synaptic Neuroscience and Technology,Istituto Italiano di Tecnologia

Capitoli

  • 00:04Titolo
  • 01:09Extracting RNA from Primary Cultures to Evaluate microRNA Knockdown Efficiency
  • 03:53Stimulation of Knocked-down Neurons Using Optogenetics
  • 07:13Results: Knockdown of P/Q-type Voltage-gated Ca2+ Channel at CA3 to CA1 Synapses
  • 08:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议提供了一个工作流, 如何将人工 microRNA 介导的 RNA 干扰与光遗传学相结合, 以刺激特定突触前 boutons 在完整的神经元回路中的选择性基因表达减少。

Tags

OptogeneticsArtificial MicroRNARNA InterferencePresynaptic FunctionNeuronal CircuitsSynaptic PhysiologyPresynaptic ProteinsNeuronal CultureGene KnockdownAcute Brain SlicesIn VivoCorticoculturingAraCRNA ExtractionChloroform

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