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人胰岛自身抗体的电化学发光测定
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Electrochemiluminescence Assays for Human Islet Autoantibodies
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人胰岛自身抗体的电化学发光测定

DOI:
10.3791/57227-v

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09:15 min

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March 23, 2018

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, , , , ,
1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Endocrinology,First Affiliated Hospital of Nanjing Medical University

Capitoli

  • 00:05Titolo
  • 01:51Labeling of the Human Islet Autoantigen Followed by Incubation of the Serum Samples with the Labeled Antigens
  • 05:01Preparation of the Streptavidin Plate, Transfer of the Serum/Antigen Incubate, and Reading of the Streptavidin Plate
  • 06:32Results: Data Analysis
  • 08:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了检测人胰岛抗体的方法, 使用电化学发光 (ECL) 测定。该协议, 用于预测1型糖尿病, 可以扩大, 以检测自身抗体的其他自身免疫性疾病。

Tags

ElectrochemiluminescenceIslet AutoantibodiesType 1 DiabetesAutoimmuneNon-radioactiveRadioassayELISAInsulin AutoantibodyBiotinSULFO-TAGSpin ColumnCheckerboard AssayPhosphate Buffered Saline

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