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利用常规 PCR 技术对柑桔叶片组织样品Candidatus liberibacter的特异和准确检测

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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

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利用常规 PCR 技术对柑桔叶片组织样品Candidatus liberibacter的特异和准确检测

DOI:

10.3791/57240-v

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09:23 min

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June 29, 2018

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Huan Chen*^1,2, Ian Arthur Palmer*¹, Jian Chen², Ming Chang², Stephen L. Thompson, Fengquan Liu, Zheng Qing Fu

¹Department of Biological Sciences,University of South Carolina, ²Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences, ³Department of Instructions and Teacher Education,University of South Carolina

Capitoli

00:04Titolo
00:49Isolation of Genomic DNA from Plant Tissue
05:04Perform PCR on Genomic DNA Samplesand Electrophoresis of the Amplified DNA II. Isolation of Genomic DNA from Plant Tissueon an Agarose Gel
07:34Results: PCR Amplification of Genomic DNA to Identify the Citrus Greening Bacterium
08:52Conclusion

Summary

Traduzione automatica

柑橘类绿化是影响全球柑橘作物的一种特殊破坏性疾病。本文介绍了一种利用 PCR 和基因组 DNA 提取柑桔叶组织的简便方法, 对柑桔绿化病原体、 Candidatus liberibacter 进行准确、准确的鉴定。

Tags

Keywords: Citrus Greening Candidatus Liberibacter Spp.PCR Detection Pathogen Identification Citrus Farming Quantitative Analysis Laboratory Procedure RNA Removal DNA Precipitation Centrifugation

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