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En enkel metode for isolering av soyabønner Protoplasts og programmet forbigående Gene Expression analyser
JoVE Journal
Biologia
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JoVE Journal Biologia
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

En enkel metode for isolering av soyabønner Protoplasts og programmet forbigående Gene Expression analyser

DOI:
10.3791/57258-v

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09:22 min

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January 25, 2018

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1Department of Biology,California State University, Northridge

Capitoli

  • 00:05Titolo
  • 00:57Protoplast Isolation
  • 04:09Protoplast Transformation
  • 06:00Protoplast Incubation and Harvesting
  • 06:46Results: Efficient Isolation of Soybean Protoplasts for Transient Gene Expression Studies
  • 08:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi utviklet en enkel og effektiv protokoll for utarbeidelse av store mengder soyabønner protoplasts å studere komplekse regulatoriske og signalnettverk mekanismer i levende celler.

Tags

Soybean ProtoplastsTransient Gene ExpressionRegulatory NetworksProtein InteractionsUnifoliate LeavesEnzyme SolutionVacuum InfiltrationProtoplast DigestionW5 SolutionNylon MeshCentrifugation

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