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Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
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Bioingegneria
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JoVE Journal Bioingegneria
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

DOI:
10.3791/57259-v

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07:52 min

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June 13, 2018

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, ,
1Tissue Engineering Research Laboratory, Department of Bioengineering, IQS-School of Engineering,Ramon Llull University, 2Hebe Biolab

Capitoli

  • 00:05Titolo
  • 00:51Self-Assembling Peptide Scaffold (SAPS) Preparation
  • 01:39Cell Suspension Preparation
  • 02:323D Cell Encapsulation into SAPS
  • 04:52Cell Viability in 3D-SAPS
  • 05:55Results: Attaining a 3D-Culture System in Self Assembling Peptide Scaffolds
  • 07:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to obtain 3D-culture systems in self-assembling peptide scaffolds to promote the differentiation of dedifferentiated human articular chondrocytes into cartilage-like tissue.

Tags

3D Cell CulturePeptide ScaffoldRAD16-ICell-cell InteractionCell-matrix InteractionCell ProliferationCell MigrationCell DifferentiationRegenerative MedicineTumor ModelsDiabetesNeurodegenerative DiseasesCell SuspensionCell ConcentrationSonicationCentrifugation

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