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微流控装置在极小空间中研究尖端生长植物细胞的伸长能力

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Bioingegneria

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JoVE Journal Bioingegneria

Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces

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微流控装置在极小空间中研究尖端生长植物细胞的伸长能力

DOI:

10.3791/57262-v

•

07:01 min

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May 22, 2018

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Naoki Yanagisawa, Nagisa Sugimoto, Tetsuya Higashiyama², Yoshikatsu Sato

¹Division of Biological Science, Graduate School of Science,Nagoya University, ²Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Capitoli

00:05Titolo
00:28Fabricating the PDMS Microdevices
02:11In Vitro Culturing of T. fournieri Pollen Tubes in the Microdevice
03:31In Vitro Culturing of A. thaliana Root Hairs in the Microdevice
04:40In Vitro Culturing of P. patens (moss) Protonemata in the Microfluidic Device
05:31Results: Microfluidic Device Design and Time-lapse Imaging of Pollen Tube Growth
06:37Conclusion

Summary

Traduzione automatica

我们描述了一种方法来调查尖端生长的植物细胞, 包括花粉管, 根毛和苔藓 protonemata, 以拉长通过极窄的差距 (约1µm) 在微流控装置。

Tags

Keywords: Microfluidic Devices Tip-growing Plant Cells Pollen Tubes Moss Protonemata Root Hairs Cell Biology PDMS Air Plasma Growth Medium Pollen Grains Pollinated Style Microscopy

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