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致心律失常心肌病患者心内膜心肌活检样间质基质细胞的分离与鉴定

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

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致心律失常心肌病患者心内膜心肌活检样间质基质细胞的分离与鉴定

DOI:

10.3791/57263-v

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09:16 min

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February 28, 2018

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Chiara Assunta Pilato*^1,2, Ilaria Stadiotti*¹, Angela Serena Maione, Valentina Saverio, Valentina Catto, Fabrizio Tundo, Antonio Dello Russo, Claudio Tondo³, Giulio Pompilio², Michela Casella*³, Elena Sommariva*¹

¹Vascular Biology and Regenerative Medicine Unit,Centro Cardiologico Monzino-IRCCS, ²Department of Clinical Sciences and Community Health,Università degli Studi di Milano, ³Heart Rhythm Center,Centro Cardiologico Monzino-IRCCS

Capitoli

00:04Titolo
01:12Cardiac Biopsy Collection and Processing
03:06Characterizing Cardiac Mesenchymal Stromal Cells (C-MSC) by Flow Cytometry
05:22Oil Red O (ORO) Staining C-MSC after Adipogenic Differentiation
07:02Results: Flow Cytometry Analysis and ORO Staining
08:27Conclusion

Summary

Traduzione automatica

本文提供了一种从致心律失常心肌病患者心内膜心肌活检样品中分离心间质基质细胞的方法。描述了它们的表征和提高脂肪分化的协议。

Tags

Cardiac Mesenchymal Stromal Cells Arrhythmogenic Cardiomyopathy Endomyocardial Biopsy Adipogenic Differentiation Phenotyping Cell Isolation Cell Culture Collagenase Digestion Surface Markers

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