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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
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Biologia
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JoVE Journal Biologia
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

DOI:
10.3791/57271-v

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06:56 min

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March 10, 2018

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, , , ,
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research,KU Leuven

Capitoli

  • 00:04Titolo
  • 00:54Competition Binding Assay
  • 03:00Analysis of the Samples by Flow Cytometry
  • 05:35Results: Evaluation and Analysis of Active and Inactive Compounds in the Competition Binding Assay
  • 06:35Conclusion

Summary

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A flow cytometry-based cellular binding assay is described that is primarily used as a screening tool to identify compounds that inhibit the binding of a fluorescently labeled CXC chemokine ligand 12 (CXCL12) to the CXC chemokine receptor 4 (CXCR4).

Tags

Flow CytometryBinding AssayCXCR4CXCL12G-protein Coupled ReceptorFluorescent LigandCompetition BindingDrug Discovery

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