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Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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Capitoli
- 00:05Titolo
- 00:39Removal of the Fur and Fixation of the Mouse on the Stage
- 02:46Surgical Exposure of the Salivary Gland Using a Stereomicroscope
- 04:09Fixing of the Gland, Heating Ring, and the Temperature Probe
- 06:10Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
- 07:19Conclusion
We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.
