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A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
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Genetica
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JoVE Journal Genetica
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

DOI:
10.3791/57289-v

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07:00 min

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April 01, 2019

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, , , , , , , , , ,
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine,University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences,University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences,University of L’Aquila, 4CNR – Institute of Molecular Genetics, Section of Chieti

Capitoli

  • 00:04Titolo
  • 00:56Reagent Setup
  • 02:33Purification and PCR Amplification of DNA Samples
  • 03:39RFLP of PCR Products Followed by Gel Electrophoresis of the PCR-RFLP Samples
  • 04:44Results: Analysis of RFLP to Identify the C924T Polymorphism in TBXA2R Gene
  • 05:33Conclusion

Summary

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In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.

Tags

C924T PolymorphismThromboxane A2 Receptor GeneAtherothrombosisPersonalized MedicinePharmacological ResponseTris EDTA BufferElectrophoresis BufferAgarose GelDNA PurificationDNA Quantification

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