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Manipulation of Ploidy in Caenorhabditis elegans
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Genetica
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JoVE Journal Genetica
Manipulation of Ploidy in Caenorhabditis elegans

Manipulation of Ploidy in Caenorhabditis elegans

DOI:
10.3791/57296-v

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07:54 min

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March 15, 2018

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, , , ,
1Brooklyn College, Biology Department,City University of New York, 2The Graduate Center, Biology Department,City University of New York, 3Advanced Science Research Center,City University of New York

Capitoli

  • 00:04Titolo
  • 01:25Setting Up rec-8 RNAi (Day 1 – 3 in Figure 2)
  • 02:32Generating and Isolating Tetraploids (Day 4 – 16 in Figure 2)
  • 03:57Verifying Tetraploid Strains using DAPI Staining
  • 05:56Results: Generation of Tetraploid C. elegans Strains
  • 07:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.

Tags

TetraploidPloidyCaenorhabditis ElegansRec-8RNAiPolyploidizationGene DosageGenome InstabilitySpeciationDiploidSpermatocytesOocytesE. ColiIPTGAmpicillinLBHermaphroditesL4 Stage

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