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Immunologia e infezioni

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小鼠胰岛细胞筛选致糖尿病环境化学物质的研究

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

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小鼠胰岛细胞筛选致糖尿病环境化学物质的研究

DOI:

10.3791/57327-v

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07:39 min

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June 25, 2018

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Jingshu Chen*², Lei Zhong*¹, Jing Wu, Sui Ke, Benjamin Morpurgo, Andrei Golovko, Nengtai Ouyang, Yuxiang Sun, Shaodong Guo, Yanan Tian²

¹Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,Hunan Agriculture University, ²Texas A&M University, ³Texas A&M Institute for Genomic Medicine, ⁴Sun Yat-Sen Memorial Hospital

Capitoli

00:04Titolo
00:49Pancreas Perfusion and Removal
01:43Pancreas Digestion and Purification of Islets
03:30Plating and Treatment of Islet Cells
04:23ROS Staining and Measurement
05:28Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
06:39Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个协议, 以隔离小鼠胰岛细胞筛选的 ROS 归纳的外来化合物, 以确定潜在的致糖尿病 xenobiotic 化学品。

Tags

Pancreas Islet Cells ROS Type-2 Diabetes In Vitro Collagenase Digestion Isolation Purification Glucose-stimulated Insulin Secretion

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