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秀丽线虫纵向成像的微流控平台
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JoVE Journal Bioingegneria
A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
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秀丽线虫纵向成像的微流控平台

DOI:
10.3791/57348-v

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09:00 min

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May 02, 2018

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,
1Department of Physics Education,Kongju National University, 2Department of Physics,Harvard University, 3FAS Center for Systems Biology,Harvard University

Capitoli

  • 00:04Titolo
  • 00:58Microfluidic Environment Preparation
  • 03:35Microfluidic Device Bacteria Loading
  • 04:53Microfluidic Device Worm Loading
  • 06:50Results: Representative Gene Expression and Egg Laying Kinetics
  • 08:08Conclusion

Summary

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在本文中, 我们演示了使用自定义微流控装置的单个蠕虫的实时成像。在该装置中, 多个蠕虫单独局限于单独的分庭, 允许多路纵向监测各种生物过程。

Tags

Keywords: Microfluidic PlatformCaenorhabditis ElegansLongitudinal ImagingEnvironmental ConditionsWorm Behavioral ResponsesGene ExpressionAdaptationMathematical ModelingHypothesis TestingSyringe TubeAdapter NeedleBuffer ExchangeS-mediumDevice OutletBuffer InletWorm Inlet

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