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Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
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Genetica
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JoVE Journal Genetica
Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

DOI:
10.3791/57351-v

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09:34 min

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May 25, 2018

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, , , , , ,
1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital,Capital Medical University, 2Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital,Shanghai Jiao Tong University

Capitoli

  • 00:05Titolo
  • 00:56Device Preparation
  • 02:31Canalostomy in Adult Mice
  • 05:58Canalostomy in Neonatal Mice
  • 06:48Results: Drug Delivery by Canalostomy Resulted in Widespread Distribution of the Reagent Throughout the Cochlea and Vestibular End-organs
  • 08:13Conclusion

Summary

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Here we describe canalostomy procedure which allows local drug delivery into the inner ears of adult and neonatal mice through the semicircular canal with minimum damage to hearing and vestibular function. This method can be used to inoculate viral vectors, pharmaceuticals, and small molecules into the mouse inner ear.

Tags

Keywords: CanalostomyInner Ear Drug DeliveryAdult MiceNeonatal MiceSurgical TechniquePolyimide TubingPolyethylene TubingInjection CannulaFast Green DyeAAV8-GFPStreptomycinMicroinjection PumpPostauricular IncisionSemicircular Canal

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