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/
Immunologia e infezioni
/
具有量身定做多样性的合成噬菌体展示库的构建
JoVE Journal
Immunologia e infezioni
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JoVE Journal
Immunologia e infezioni
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Please note that all translations are automatically generated.
Click here for the English version.
具有量身定做多样性的合成噬菌体展示库的构建
DOI:
10.3791/57357-v
•
12:31 min
•
May 01, 2018
•
Ganggang Huang*
1
,
Zhenwei Zhong*
1
,
Shane Miersch
3
,
Sachdev S. Sidhu
2,3
,
Shin-chen Hou
,
Donghui Wu
1
Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies
,
ShanghaiTech University
,
2
Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research
,
University of Toronto
,
3
Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research
,
University of Toronto
Capitoli
00:04
Titolo
01:18
Electroporation and Calculation of the Library Size
06:02
Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
09:43
Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
11:07
Conclusion
Summary
Traduzione automatica
English (Original)
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Traduzione automatica
本议定书描述了一个详细的程序, 以构建一个噬菌体显示合成抗体库, 具有量身定做的多样性。合成抗体具有广泛的应用, 从基础研究到疾病诊断和治疗。
Tags
Synthetic Phage Display
Fab Library
Antibody Library Construction
Antibody Diversity
Monoclonal Antibody Development
Phage Display Technology
Kunkel Mutagenesis
Electroporation
Diversity Evaluation
ELISA
Article
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