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Bioengineering

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JoVE Journal Bioengineering
Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
 

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Article DOI: 10.3791/57363-v • 08:43 min • February 7th, 2018
February 7th, 2018 •
1, 1, 1,2, 3, 1,2
1Department Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg, 2Translational Center Regenerative Therapies (TLC-RT), Fraunhofer Institute for Silicate Research ISC, 3Fujifilm Manufacturing Europe B.V.

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We propose a cell expansion protocol on macroporous microcarriers and their use as delivery system in a perfusion bioreactor to seed a decellularized tissue matrix. We also include different techniques to determine cell proliferation and viability of cells cultured on microcarriers. Furthermore, we demonstrate functionality of cells after bioreactor cultures.

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Keywords: Recombinant Collagen I Peptide Microcarriers Cell Expansion Cell Delivery Bioreactor Tissue Engineering Vascularization Spinner Flask Macroporous Microcarriers Dulbecco's Phosphate Buffered Saline Autoclave Culture Medium Human Bone Marrow Derived Cells
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